DNA cloning is one of the ways to create many identical copies of a piece of DNA, for example, a gene. In a classic cloning experiment, the target gene is inserted into a circular DNA molecule called a plasmid. The plasmid is introduced into bacteria through a process called transformation. Bacteria carrying the plasmid are…
Diabetes mellitus is a whole group of diseases associated with a chronic increase in blood glucose levels, which is caused by insufficient production of insulin or its ineffective action. The risk of developing diabetes mellitus depends on many factors. In type 2 diabetes mellitus, the process of glucose uptake by cells is disrupted, since the…
Our modern idea of DNA and heredity has led to the development of a large number of trends, such as forensic analysis, paternity testing and genetic screening. Therefore, thanks to such widespread use, today many people have the basic idea of what DNA is. Proteins and DNA According to Gregor Mendel signs (such as painting…
Bioinformatics is an interdisciplinary field that combines general biology, molecular biology, cybernetics, genetics, chemistry, computer science, mathematics and statistics. Large-scale biological problems requiring the analysis of large amounts of data are solved by bioinformatics from a computational point of view. Bioinformatics mainly includes the study and development of computer methods and is aimed at obtaining,…
DNA microchips are used to identify genes and their mutations associated with various diseases; monitoring of gene activity; diagnosis of infectious diseases and determining the most effective antibiotic therapy method; screening microorganisms. DNA microarrays, in contrast to real-time PCR technologies or mass spectrometry, allow you to analyze many mutations in one person at the same…
The Affymetrix platform is a comprehensive solution for high-throughput genetic analysis based on the hybridization of fluorescently-labeled genome fragments with oligonucleotide DNA probes attached to a solid support (DNA microarray technology). These solutions are widely used for basic and applied research of DNA (for genotyping) and RNA (for analyzing gene expression). DNA chip format: cartridges,…
First you need to understand the terms. To begin with, genetic diseases and diseases to which a hereditary predisposition has been identified are different concepts. Genetic diseases are caused by abnormalities in the structure of the genome (hence the other name – monogenic diseases). An example is galactosemia. In this disease, enzymes that convert milk…
1. Opening an account Complete the Sequencing Request Form Press “submit”, it will be sent to us by email. You will receive a password and user name to access Nanuq, our web application. You will then be able to submit your samples online. If any of the samples to be sequenced in your project are directly or…
PCR is a laboratory diagnostic method aimed at identifying the causative agents of infectious diseases. The three-letter version is an abbreviation of the name “polymerase chain reaction”. Actually, this name reflects the essence of the method, but in order to understand it, you will have to thoroughly recall the school course in biology. But first,…